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KMID : 0613820010110020190
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Journal of Life Science
2001 Volume.11 No. 2 p.190 ~ p.195
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Expression Characteristics of Recombinant Cyclodextrin Glucanotransferase in Saccharomyces cerevisiae
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Jun Hyun-Sung
Nam Soo-Wan
Kim Byung-Woo
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Abstract
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The cuclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subcloned at the downstream of yeast ADH1 promoter, and then the resulting plasmid pVT-CGTM(9.15 kb) was introduced into the yeast host strain, Saccharomyces cerevisias 2805. The transformed yeast, S. cerevisiae 2805/pVT-CGTM, showed the starch-hydrolyzing activity on the starch-azure plate. The optimal conditions for the CGTase expression were found to be 2% dextrose, initial pH5.5, 30^{circ}C, and 48hr cultivation. Under this condition, the extracellular CGTase activity reached at 0.53 U/mL, whereas the intracellular activity was about 0.03U/mL. This result indicates that the signal peptide of Bacillus CGTase functioned well in S. cerevisiae.
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KEYWORD
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Bacilus macerans, cuclodextrin glucanotransferase, Saccharomyces cerevisiae, secretion
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